Trajectory outputs - mPFC reference
1.13mm AP entry offset5.42mm insertion length-4.40mm corrected DV0.50mm craniotomy
10 deg approach. Verify second manipulator clearance.
VTA dopamine optogenetics - Cohort 24-Bplan_8fr2k9 - saved 14:12draft
Step 2 / 6 - Targets
Step 2 - Coordinate planner
Targets, bregma-zeroed.
Atlas: Paxinos and Franklin, 5th ed. Bregma is the operative reference; lambda delta verified within 100 um.
Skull leveling
Bregma to lambda delta on DV axis
-4.20 mm0.04 mmLevel - within 100 um| Target | Hemi | AP | ML | DV | Angle | Device | Inj. vol | Dwell | Status |
|---|---|---|---|---|---|---|---|---|---|
| VTA - left | left | -3.20 | -0.50 | -4.40 | 0 | Fiber cannula | 0.50 uL | 10 min | ok |
| VTA - right | right | -3.20 | +0.50 | -4.40 | 0 | Fiber cannula | 0.50 uL | 10 min | ok |
| mPFC reference | right | +1.95 | +0.35 | -2.30 | 10 | Hamilton 33ga | 0.30 uL | 5 min | review |
Equipment readiness
Digital mouse stereotaxic, dual-armFrame - calibratedready
Mouse incisor and ear bar adapter setSubject fixation - fit checkedready
Gas anesthesia mask and scavengerAnesthesia - bench readyready
Mono fiber-optic cannula holder (200 um)Optogenetics - verify fit before surgeryverify
Micro-drill and drill holderCraniotomy - bench readyready
Checklist preview
Sterilize stereotaxic frame and manipulator arms.
Zero digital readouts on all axes before mounting subject.
Locate bregma and record DV reference.
Verify lambda delta within 100 um before target entry.
Confirm VTA-left and VTA-right coordinates before drilling.
Hold dwell time before withdrawal and export the coordinate table.
Export package
Ready for review.
Setup summary, coordinate table, trajectory warning, equipment readiness, and checklist state are included.
Fit check: Mono fiber-optic cannula holder should be verified before this plan is marked ready.
Planning aid only. Verify all coordinates, device settings, anesthesia, analgesia, and surgical steps against your approved protocol, atlas edition, equipment manual, and institutional animal-care requirements.